Outreach activities of the Extreme Energy Events Project

The Extreme Energy Events Project (EEE) represents a breakthrough in outreach activities in Cosmic Ray Physics: high school students are protagonists of an experiment to measure Extensive Air Showers at ground. They start their experience at CERN with the construction of the three high performing Multigap Resistive Plate Chambers constituting the telescope that is then installed inside their school;then they take care of the telescope operation and data analysis. Presently 60 telescopes are installed in Italy and, since 2014, coordinated data taking have been performed during each school year providing a huge amount of candidate muon tracks. Every year hundreds of students and teachers are involved in the activities directly correlated to EEE. The COVID-19 pandemic has strongly affected the experimental activities of the EEE Project. However in the last two years the online activities were strengthened, with an intense programme of collaboration meetings, masterclasses, and hugely successful topical seminars. Starting from the fall of 2021, the improvement of epidemiological situation made it possible to start some of the EEE activities in presence. © Copyright owned by the author(s) under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0)

Outreach and educational activities within the EEE cosmic ray network

The Extreme Energy Events (EEE) network consists in a sparse array of telescopes based on Multigap Resistive Plate Chambers, installed in high school buildings all over the Italian territory and at CERN. Besides the many research activities concerned with extensive air shower detection, long distance correlation studies and additional physics results obtained during the last decade, the EEE project is extensively employed for educational and outreach activities, constituting a unique opportunity to promote a fruitful and close collaboration between students, high-school teachers and researchers. The involvement is at all levels, from the construction of the chambers during short stages at CERN over the past 15 years, with the participation of several hundred high-school students and teachers, to the installation, monitoring and data taking with the telescopes by high-school teams, to masterclasses, physics lectures, data analysis sessions and joint discussions on the results and their interpretation. Recent developments of the EEE network led to the installation and use of additional detectors in the Arctic region and on board of sailing ships, to measure the cosmic ray flux over large latitude intervals. Periodical remote and in presence (pre-Covid era) meetings allowed in these years a large participation (several thousand people) from the high-school community to the EEE activities. National and local outreach initiatives in cosmic ray physics are also carried out around Italy by the EEE network, as a contribution to the dissemination of science among young people. © Copyright owned by the author(s) under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0)

Contribution of mast cells in SARS-CoV-2-induced inflammation

COVID-19, which first appeared in China, has so far caused an unexpected number of deaths, as our immune system has not been able to annihilate the SARS-CoV-2 virus. SARS-CoV-2 reacts to both innate and acquired immunity. In the first instance, when the virus enters our organism, it is attacked by innate immune cells, including macrophages and mast cells (MCs), which produce defensive cytokines such as IL-1, IL-6, IL-33 and TNF;but the overproduction of these cytokines is very harmful to the patient. Here, in this editorial, we report that the inflammatory cytokine network established in COVID-19, in the most serious cases, can lead to the death of the patient. Therefore, it is pertinent to think that by blocking the pro-inflammatory cytokines that cause the “cytokine storm”, a great therapeutical benefit can be achieved for COVID-19 disease.

The Extreme-Energy Events Project: a bridge between school and professional research

The Extreme-Energy Events (EEE) Experiment is a cosmic ray observatory based on a network of detecting stations distributed over the Italian territory and at CERN. A station of the network, called “telescope”, consists of three superimposed Multi-gap Resistive Plate Chambers (MRPCs), each one covering a surface of about 1.5 m2, used with the same technology as the time-of-flight detector of the ALICE experiment at CERN-LHC. Data are collected and sent in real time to the INFN-CNAF computer center for reconstruction and analysis. The large area covered by the network, ranging from Southern Italy up to the CERN laboratories, was achieved with the decision to install the detectors inside high schools, involving students and teachers in a modern experiment within a unique program. This very coexistence of scientific activity and outreach represents the uniqueness of the EEE Project. The outreach programme is articulated in several initiatives, each encoding the different aspects of the research activity normally expected in a high-energy physics experiment. Students are involved in detector construction at CERN, installation in school, and in the commissioning of the station when data taking starts. Once the detector reaches a steady working regime, students are requested to monitor on a daily basis the performance of the telescope and report any failure. In parallel to the hardware-related operations, students learn how to perform the analysis of EEE data under the supervision of their teachers and of the EEE researchers, supporting the scientific output of the experiment. Every month students report progress and issues in a dedicated online meeting open to all schools and to the EEE researchers. Beside this monthly appointment, in the pre-COVID era an in-person meeting was taking place twice per year, hosted by the Ettore Majorana Foundation and Centre for Scientific Culture in Erice or by a school or institution involved in the project. During a three-day, students attend masterclasses and take part in measurement campaigns, disseminating their results by submitting contributions to important outreach-oriented journals (such as the Italian Giornale di Fisica). © Copyright owned by the author(s)

Mast cells and virus

Mast cells (MCs) are hematopoietic cells that reside ubiquitously in all vascularized tissues. They are potential sources of a wide variety of biologically active secreted compounds, including diverse cytokines, chemokines and growth factors. In addition, they participate in innate and adaptive immune responses. MCs are the most important cells in immediate reactions and chronic IgE-associated allergic disorders and enhance the host resistance to certain biological agents, including viruses. Therefore, MCs influence many biological responses to viruses and other microbiological agents. Viruses activate MCs through TLR4 leading to the generation of several pro-inflammatory cytokines, including those of the IL-1 family. Here, we report how viruses can activate MCs producing severe inflammation and how these interesting cells can activate the immune system by carrying out a protective action for our organism.

Mast cell virus infection and inflammatory cytokines

Mast cells (MCs) are hematopoietic cells developed from bone marrow progenitors in response to the ligand stem cell factor, a trans-membrane tyrosine kinase kit receptor. MCs are located virtually in all vascularized tissues and in proximity to neurons and play a decisive role in both innate and adaptive immune responses. Their activation is involved in oxidative stress correlated with infection and inflammation. Pro-inflammatory cytokines are secreted by MCs after physiologic and psychological stress due to virus infection, including SARS-CoV-2. MCs, along with macrophages and pulmonary alveolar epithelial cells, are the main targets attacked by the coronavirus. COVID-19 induced by SARS-CoV-2 causes inflammatory stress which activates MCs to secrete corticotrophin-releasing hormone (CRH), SP, IL-6, TNF, and IL-1. Toll-like receptor (TLR) virus activation in MCs leads to pro-inflammatory cytokine generation without degranulation, an effect that can be inhibited by IL-10, IL-4, IL-1Ra and IL-37. TLR has the ability to recognize extracellular PAMPs by causing the transcription of NLRP, pro-IL-1, and other pro-inflammatory cytokines. The multi-protein complex, comprising pro-caspase-1, activates caspase-1 which in turn activates pro-IL-1 that is transformed into highly inflammatory mature IL-1. In COVID-19, viral RNA is specifically recognized by TLR, followed by recruiting the signal transfer proteins MyD88, IRAK, IKK and TRAF6 which can activate the NF-κB, resulting in transcription of the pro-inflammatory cytokines IL-1 and TNF, responsible for the “cytokine storm” phenomenon. Meanwhile, a new variant of the coronavirus-19 called C.1.2. has been discovered in the United States in the past few days, the effects of which are unknown, and it is therefore of great concern. Researchers are now testing it on immune cells to see if they react and are comparing it to a delta variant. Thus, from the existing data in biomedical literature, we can conclude that the suppression of pro-inflammatory cytokines in viral infections (including COVID-19) mediated by MCs represents a promising therapy not only in this field of medicine, but also in autoimmune, allergic, and cardiovascular disorders, as well as tumor inflammation where MCs play a key role.

Covid-19 and the reform of local government in Italy

The administrative response to covid-19 has shown the inadequacy of the local government system organised into an inordinate number of municipalities, many of which are very small. Another aspect that has emerged is the municipal response to the pandemic, marked by the adoption of municipal ordinances of doubtful compatibility with constitutional lawfulness. Reflecting on these elements, this article quickly identifies some possible prospects for reforming municipal autonomy in Italy. © 2020, University of Urbino. All rights reserved.

Acetic acid disinfection as a potential adjunctive therapy for non-severe COVID-19.

PURPOSE: SARS-CoV-2 is a new pandemic influenza caused by a coronavirus which main route of transmission is through exhaled droplets that primarily infect the nose and the nasopharynx. The aim of this paper is to evaluate the effect of acetic acid, the active component of vinegar, as a potential disinfectant agent for upper airways. METHODS: Twenty-nine patients were enrolled and divided into two groups: group 1 (14 patients) was composed of patients treated with off-label hydroxychloroquine and lopinavir/ritonavir, whereas group 2 (15 patients) was composed of patients treated with hydroxychloroquine only, combined with the inhalation of acetic acid disinfectant at a 0.34% concentration. A questionnaire-based evaluation of symptoms was performed after 15 days in both groups. RESULTS: It appears that the number of patients treated with acetic acid (group 2) that experienced improvement in individual symptoms was double that of the other group of patients (group 1), although numbers are too small for robust statistical analysis. CONCLUSIONS: Considering its potential benefits and high availability, acetic acid disinfection appears to be a promising adjunctive therapy in cases of non-severe COVID-19 and deserves further investigation.